Vorinostat (SAHA, MK0. A, q. RT- PCR of human IL- 1. R. Tumors were harvested next day after IL- 1.

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PE treatment and total RNA extracted. Data shown is ratio of human IL- 1. R. Bars , SD of triplicate determinations.

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B, q. RT- PCR of mouse IL- 1. R. Tissues were harvested at the same time point as in panel A and total RNA extracted.

Data shown is ratio of mouse IL- 1. R. C, IHC of human IL- 1. R. D, IHC of mouse IL- 1.

R. Liver, brain, kidney, pancreas, lung and spleen were fixed for immunostaining of mouse IL- 1. R. Nucleus was counterstained by DAPI.

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